LNEG-BBRI2

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LNEG-BBRI – Fermentation-based Biorefineries

TA71 – LNEG-BBRI2 

Location

Estrada do Paço do Lumiar, 22

1649-038 Lisboa,  Portugal 

Description

TA71 is the Research Infrastructure (RI) offered at LNEG (called BBRI-Biomass and Bioenergy Research Infrastructure) belonging to the Portuguese Roadmap of Research Infrastructures. The main aim of TA71 LNEG-BBRI is the development of biomass processing technologies for different end-uses (energy-based and bio-based products), the promotion of research excellence, external services provider, and training of new professionals on sustainable bioenergy and bioeconomy.

TA71-BBRI2 is offering research and development (R&D) and advanced training activities on Fermentation-based Biorefineries to the scientific community, professionals, universities, R&D institutes and industry. The activities will be directed towards professionals working in the biomass and bioenergy markets and MSc, PhD and Post-Doc students aiming at increasing the number of professional experts on Fermentation-based Bioprocesses. These activities offered by TA71-BBRI2 dealing with the development and implementation of integrated Fermentation-based Biorefineries can include upstream and downstream processes coupled to Fermentation Technology, such as biomass fractionation and pre-treatment, enzyme technology and downstream processing.

Testing Capabilities

The infrastructure supports the development of integrated microbial-based bioprocesses for sustainable and cost-efficient processes for production of advanced biofuels or bio-based products, using mainly lignocellulosic wastes and by-products as alternative raw materials.

Lignocellulosic biomass can firstly be processed by steam explosion (at pilot scale) as pre-treatment, and thereafter subject to enzymatic hydrolysis for the liberation of sugars that will be used in the fermentation stage. Depending on the process to be implemented, the microbial conversion might be run under aerobic or anaerobic conditions, implemented under batch, fed-batch or continuous mode. The best process integration, including pre-treatment, enzymatic hydrolysis and fermentation, can be targeted. Downstream processing by membrane-separation can also be integrated aiming towards, for example, in-situ product recovery, providing operation as a membrane bioreactor.

Technical Equipment

  • Bench-scale (1-7 L) and pilot scale (100 L) fermenters, including all the accessories for monitoring and control, and a workstation for cultivation of anaerobic microorganisms.

  • Lab-scale (5 L) and pilot scale (up to 1000 L) bioreactors specifically designed to run enzymatic hydrolysis (including pre-liquefaction)

  • Pilot-scale steam explosion equipment

  • Several (bench and pilot-scale) membrane separation systems for DSP – microfiltration, ultrafiltration, nanofiltration, reverse osmosis and pervaporation

  • Analytical equipment such as: physiological and molecular monitoring with multiparametric flow cytometer and PCR; chemical analysis via high-performance liquid chromatography (HPLC), ( equipped with diode array detector (DAD) and refractive index (RI) DAD- and RI detectors) and gas chromatography (GC), ( equipped with flame ionisation detector (FID) and thermal conductivity detector (TCD) systems

Additional information

Technology Readiness Level: 4-6

Special considerations: N/A 

Technology clusters: Biofuels, Materials for Energy, Others

Website: https://www.lneg.pt/en/infrastructure/bbri-biomass-and-bioenergy-research-infrastructure-2/

Availability: All year

Provision of tools to prepare data sets in a FAIR way:  Yes 

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